Paper tawol seed germination method practical video

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PAPER TAWOL SEED GERMINATION METHOD

PRACTICAL BY-DHANENDRA DHANUKA

Science Lab Report:  Seed Germination Experiment

 

  1. Problem: What is the effect of acid on the seed germination of green bean seeds?

 

  1. Research: This section should be a minimum of two paragraphs and include information about your particular seed, what seeds germination is, what the requirements for germination are, etc.

 

  1. Hypothesis: If the seeds are treated with an acidic solution with a pH of 5, then 50% of the seeds will germinate.

 

  1. Experiment:

A.  Materials– two paper towels of the same size, two Zip Loc gallon size freezer bags, 20 green bean seeds, a solution of vinegar with a pH of 5, distilled water, pipet, and masking tape

B. Variables

1. Independent (manipulated) variable: using an acid solution with a pH of 5     to treat seeds

2. Dependent (responding) variable: seed germination rate (%)C.

Procedure:       

1.  Gather all of the materials needed to perform the experiment.

2.  Make the acid solution by diluting household vinegar with water     until a pH of 5 is obtained.

3.  Label each Zip Loc bag with the treatment type, name of person in lab group, and period number.

4.  Place 10 of the green bean seeds on the paper towel so that they are equally spaced and above the fold of the paper towel.

5.  Treat each seed with 1 mL of the acid solution (pH of 5). (Experimental group)

6.  Seal the bag and place on the left side of the classroom on the windows with masking tape.               7.  Place the other 10 green bean seeds in the second paper towel so that they are equally spaced and above the fold of the paper towel.

8.  Treat each of these seeds with 1 mL of water only.  (Control group)

9.  Seal the bag and place on the left side of the classroom on the windows with masking tape next to the bag that was treated with acid.  This will ensure that both bags are exposed to the same amount of light and temperature. (Controlled variables)

10. Record the seed germination rate and other observations in both bags over a seven day period.

Data Collection/Analysis   A.  DATA TABLE:

Table 1.  Germination data for seeds in Control Group (1 mL of water per seed)
Date Started Days since start Total Seeds in Treatment # Seeds Germinated During Time Interval Total Seeds Germinated Since To Percent Germination during time interval (per day) Cumulative Percent Germination
0  10 0 0 0 0
1  10 0 0 0 0
2  10 0 0 0 0
3  10 3 3 30 30
4  10 3 6 30 60
5  10 1 8 20 80
6  10 1 9 10 90

Table 2.  Germination data for seeds in Experimental Group (1 mL of acid per seed)

Date Started Days since start Total Seeds in Treatment # Seeds Germinated During Time Interval Total Seeds Germinated Since To Percent Germination during time interval (per day) Cumulative Percent Germination
0  10  0  0 0 0
1  10  0  0 0 0
2  10  0 0 0 0
3  10  0 0 0 0
4  10  0 0 0 0
5  10  0 0 0 0
6  10  0 0 0 0

 B. CALCULATIONS:

To calculate the germination rate, I took the number of seeds that germinated during that time and divided by 10.

This number was then multiplied by 100 to obtain the % of seed germination during that time period.  The cumulative seed germination percentage was calculated by adding all of the percentages at that point during the experiment.

 C. VISUAL REPRESENTATION OF DATA

6. CONCLUSION:

My hypothesis was not supported.  I hypothesized that the overall seed germination percentage would be 50% when treated with acid.

The data shows that none of the seeds germinated when treated with acid.  Those treated with water had an overall seed germination percentage of 90% during the course of the experiment.

The seeds in the control group germinated better over time that those in the experimental group.

7. Repeat and/or redesign the experiment           

    I believe that my data is valid.  I carefully designed my experiment to make sure that the only thing that changed was placing acid on the seeds that were in the experimental group.

We made sure that the seeds were placed in the same amount of light and temperature by placing them in the same place in the room.

The seed germination-

:bags were also made in an identical manner; the only difference being that one bag received 1 mL of acid per seed while the other received 1 mL of water per seed.

Three possible sources of error during this experiment may have been that the temperatures fluctuated in the classroom from one day to the next.

Hopefully, this problem was not a factor due to both bags being in the same place.  The seeds may have also not received the proper amount of sunlight needed for germination because three days of the experiment were relatively cloudy days.

Another factor may have been that the seeds had a tendency to slip out of their proper positions.  This may have affected the space that they need to germinate properly.

I believe that this experiment could be improved by folding the paper towels in a manner which allows them to slide into and out of the bags easier.

I also think that more than one pH should have been tested to see what the best pH for seed germination is.

This will be beneficial because you would know what pH the soil needs to be in order for the seed to germinate best.

Another way to improve the experiment would be to use grow lights so that the temperature and light fluctuations that occur in the classroom would not affect the experiment.   

 

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